原标题：EB病毒DNA载量在EBV相关性疾病中临床应用：队列研究
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Objectives
This study evaluated the diagnostic value of Epstein-Barr virus (EBV) DNA load in blood samples of patients with EBV-associated diseases, and proposed a strategy for the interpretation of positive EBV DNA results.
目的：本研究评估了EB病毒相关性疾病患者血标本中EBV 病毒载量的诊断价值，提出一种方法可以更好诠释EBV DNA阳性结果。
Methods
Derivation and validation cohorts were established to evaluate the clinical significance of EBV DNA loads in the peripheral blood mononuclear cells (PBMCs) and plasma from EBV-infected patients. EBV DNA loads were compared and receiver operating characteristic curves were employed to assess the optimal cutoff values of EBV DNA for identification of EBV-associated diseases.
Results
The derivation and validation cohorts comprised 135 and 71 subjects, respectively. EBV DNA loads in the PBMCs of the EBV-associated diseases group was significantly higher than that of the EBV non-associated diseases group (5.8 × 10 4 vs7.8 × 10 3 copies/10 6 cells, P<0.0001). The diagnostic cut-off value of viral load in PBMCs for EBV-associated diseases was determined to be 1.6 × 10 4 copies/10 6 cells. The combined EBV DNA load cutoff in PBMCs and positive EBV DNA qualitative detection in plasma ( >500 copies/mL) allowed for the differentiation of EBV-associated and non-associated diseases; the sensitivity and specificity were 80.6 and 96.8%, respectively.
结果：建模队列和验证队列分别由135名和71名受试者组成。EBV相关性疾病组PBMCs中EBV DNA载量明显高于EBV非相关疾病组（5.8×10^4 vs 7.8×10^3拷贝/10^6细胞，P<0.0001）。EBV相关性疾病的PBMCs中EBV病毒载量的cut-off值为1.6×10^4拷贝/10^6细胞。联合PBMCs EBV病毒载量cut-off值（1.6 × 10^4拷贝/10^6细胞）和血浆中EBV阳性的定性检测（>500拷贝/mL），用于从非相关性疾病中鉴别EBV相关性疾病的敏感性和特异性分别为80.6和96.8%
Conclusions
The strategy of combining EBV DNA loads in PBMCs and plasma will potentially help identify EBV-associated diseases.
参考文献：Yu Shenglei,Yang Qingluan,Wu Jing,Zhu Mengqi,Ai Jingwen,Zhang Haocheng,Xu Bin,Shao Lingyun,Zhang Wenhong. Clinical application of Epstein-Barr virus DNA loads in Epstein-Barr virus-associated diseases: A cohort study[J]. Journal of Infection,2020(prepublish).
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END
供稿 /沈泳利
审核 /胡南南 翁亚丽
排版 /黄雨欣
责任编辑 / 史萍
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